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Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
The role of thymidylate synthase (TS) is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study, TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stag...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170281/ https://www.ncbi.nlm.nih.gov/pubmed/21824439 http://dx.doi.org/10.1186/1756-8722-4-33 |
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author | Liu, Yanyan Xia, Qingxin Jia, Yanzhao Guo, Hongqiang Wei, Bing Hua, Yawei Yang, Shujun |
author_facet | Liu, Yanyan Xia, Qingxin Jia, Yanzhao Guo, Hongqiang Wei, Bing Hua, Yawei Yang, Shujun |
author_sort | Liu, Yanyan |
collection | PubMed |
description | The role of thymidylate synthase (TS) is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study, TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stage II/III colorectal cancer by laser capture microdissection and polymerase chain reaction. Thirty (39.47%) patients were found to have higher TS expression in primary tumors with earlier stage (P = 0.018), lower histological grades (P = 0.001) and high frequency microsatellite instability (P = 0.000). Multivariate analysis showed that microsatellite instability, histological grade and number of lymph nodes examined are independent prognostic markers. |
format | Online Article Text |
id | pubmed-3170281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31702812011-09-10 Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer Liu, Yanyan Xia, Qingxin Jia, Yanzhao Guo, Hongqiang Wei, Bing Hua, Yawei Yang, Shujun J Hematol Oncol Letter to the Editor The role of thymidylate synthase (TS) is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study, TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stage II/III colorectal cancer by laser capture microdissection and polymerase chain reaction. Thirty (39.47%) patients were found to have higher TS expression in primary tumors with earlier stage (P = 0.018), lower histological grades (P = 0.001) and high frequency microsatellite instability (P = 0.000). Multivariate analysis showed that microsatellite instability, histological grade and number of lymph nodes examined are independent prognostic markers. BioMed Central 2011-08-09 /pmc/articles/PMC3170281/ /pubmed/21824439 http://dx.doi.org/10.1186/1756-8722-4-33 Text en Copyright ©2011 Liu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Letter to the Editor Liu, Yanyan Xia, Qingxin Jia, Yanzhao Guo, Hongqiang Wei, Bing Hua, Yawei Yang, Shujun Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer |
title | Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer |
title_full | Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer |
title_fullStr | Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer |
title_full_unstemmed | Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer |
title_short | Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer |
title_sort | significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170281/ https://www.ncbi.nlm.nih.gov/pubmed/21824439 http://dx.doi.org/10.1186/1756-8722-4-33 |
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