Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer

The role of thymidylate synthase (TS) is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study, TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stag...

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Autores principales: Liu, Yanyan, Xia, Qingxin, Jia, Yanzhao, Guo, Hongqiang, Wei, Bing, Hua, Yawei, Yang, Shujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170281/
https://www.ncbi.nlm.nih.gov/pubmed/21824439
http://dx.doi.org/10.1186/1756-8722-4-33
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author Liu, Yanyan
Xia, Qingxin
Jia, Yanzhao
Guo, Hongqiang
Wei, Bing
Hua, Yawei
Yang, Shujun
author_facet Liu, Yanyan
Xia, Qingxin
Jia, Yanzhao
Guo, Hongqiang
Wei, Bing
Hua, Yawei
Yang, Shujun
author_sort Liu, Yanyan
collection PubMed
description The role of thymidylate synthase (TS) is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study, TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stage II/III colorectal cancer by laser capture microdissection and polymerase chain reaction. Thirty (39.47%) patients were found to have higher TS expression in primary tumors with earlier stage (P = 0.018), lower histological grades (P = 0.001) and high frequency microsatellite instability (P = 0.000). Multivariate analysis showed that microsatellite instability, histological grade and number of lymph nodes examined are independent prognostic markers.
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spelling pubmed-31702812011-09-10 Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer Liu, Yanyan Xia, Qingxin Jia, Yanzhao Guo, Hongqiang Wei, Bing Hua, Yawei Yang, Shujun J Hematol Oncol Letter to the Editor The role of thymidylate synthase (TS) is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study, TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stage II/III colorectal cancer by laser capture microdissection and polymerase chain reaction. Thirty (39.47%) patients were found to have higher TS expression in primary tumors with earlier stage (P = 0.018), lower histological grades (P = 0.001) and high frequency microsatellite instability (P = 0.000). Multivariate analysis showed that microsatellite instability, histological grade and number of lymph nodes examined are independent prognostic markers. BioMed Central 2011-08-09 /pmc/articles/PMC3170281/ /pubmed/21824439 http://dx.doi.org/10.1186/1756-8722-4-33 Text en Copyright ©2011 Liu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Letter to the Editor
Liu, Yanyan
Xia, Qingxin
Jia, Yanzhao
Guo, Hongqiang
Wei, Bing
Hua, Yawei
Yang, Shujun
Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
title Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
title_full Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
title_fullStr Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
title_full_unstemmed Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
title_short Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
title_sort significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170281/
https://www.ncbi.nlm.nih.gov/pubmed/21824439
http://dx.doi.org/10.1186/1756-8722-4-33
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