Mapping protein interactions by combining antibody affinity maturation and mass spectrometry

Mapping protein interactions by immunoprecipitation is limited by the availability of antibodies recognizing available native epitopes within protein complexes with sufficient affinity. Here we demonstrate a scalable approach for generation of such antibodies using phage display and affinity maturat...

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Detalles Bibliográficos
Autores principales: Dyson, Michael R., Zheng, Yong, Zhang, Cunjie, Colwill, Karen, Pershad, Kritika, Kay, Brian K., Pawson, Tony, McCafferty, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171153/
https://www.ncbi.nlm.nih.gov/pubmed/21704603
http://dx.doi.org/10.1016/j.ab.2011.05.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171153/
https://www.ncbi.nlm.nih.gov/pubmed/21704603
http://dx.doi.org/10.1016/j.ab.2011.05.005

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