Cargando…
Mapping protein interactions by combining antibody affinity maturation and mass spectrometry
Mapping protein interactions by immunoprecipitation is limited by the availability of antibodies recognizing available native epitopes within protein complexes with sufficient affinity. Here we demonstrate a scalable approach for generation of such antibodies using phage display and affinity maturat...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171153/ https://www.ncbi.nlm.nih.gov/pubmed/21704603 http://dx.doi.org/10.1016/j.ab.2011.05.005 |