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Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli
BACKGROUND: The λ Red recombineering technology has been used extensively in Escherichia coli and Salmonella typhimurium for easy PCR-mediated generation of deletion mutants, but less so in pathogenic species of E. coli such as EHEC and EPEC. Our early experiments with the use of λ Red in EHEC and E...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2003
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC317293/ https://www.ncbi.nlm.nih.gov/pubmed/14672541 http://dx.doi.org/10.1186/1471-2199-4-11 |