Cargando…
Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease
The cytotoxic compound hydroxyurea (HU) is effective therapy for sickle cell disease. However, its effect on unsaturated membrane lipids is unknown. Red cell fatty acids were investigated in HU-treated (n = 19) and HU-untreated (n = 17) sickle cell patients and controls (n = 20). The HU-treated comp...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173880/ https://www.ncbi.nlm.nih.gov/pubmed/21941660 http://dx.doi.org/10.1155/2011/718014 |
_version_ | 1782212004658806784 |
---|---|
author | Daak, A. A. Ghebremeskel, K. Elbashir, M. I. Bakhita, A. Hassan, Z. Crawford, M. A. |
author_facet | Daak, A. A. Ghebremeskel, K. Elbashir, M. I. Bakhita, A. Hassan, Z. Crawford, M. A. |
author_sort | Daak, A. A. |
collection | PubMed |
description | The cytotoxic compound hydroxyurea (HU) is effective therapy for sickle cell disease. However, its effect on unsaturated membrane lipids is unknown. Red cell fatty acids were investigated in HU-treated (n = 19) and HU-untreated (n = 17) sickle cell patients and controls (n = 20). The HU-treated compared with the HU-untreated patients had lower arachidonic (AA) acid level in ethanolamine, physphoglycerids (EPG) (22.9 ± 1.2 versus 24.0 ± 1.1%, P < 0.05) serine SPG (22.13 ± 2.2 versus 24.9 ± 2.3%, P < 0.01) phosphoglycerides. The treated patients and controls had comparable levels of docosahexaenoic (DHA) and total n-3 fatty acids in EPG and choline phosphoglycerides (CPG). In contrast, the untreated group had significantly (P < 0.05) lower DHA and total n-3 compared with the controls in EPG (2.7 ± 0.4 versus 3.2 ± 0.6% and 4.6 ± 0.5 versus 5.2 ± 0.7%) and CPG (0.7 ± 0.2 versus 1.0 ± 0.2% and 1.2 ± 0.2 versus 1.4 ± 0.3). HU is known to activate cytosolic phospholipase A2 and cyclooxygenase 2, and from this study, it appears to induce mobilisation of AA from the inner cell membrane EPG and SPG. Hence, eicosanoids generated from the released AA may play a role in clinical improvements which occur in HU-treated patients. |
format | Online Article Text |
id | pubmed-3173880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31738802011-09-22 Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease Daak, A. A. Ghebremeskel, K. Elbashir, M. I. Bakhita, A. Hassan, Z. Crawford, M. A. J Lipids Research Article The cytotoxic compound hydroxyurea (HU) is effective therapy for sickle cell disease. However, its effect on unsaturated membrane lipids is unknown. Red cell fatty acids were investigated in HU-treated (n = 19) and HU-untreated (n = 17) sickle cell patients and controls (n = 20). The HU-treated compared with the HU-untreated patients had lower arachidonic (AA) acid level in ethanolamine, physphoglycerids (EPG) (22.9 ± 1.2 versus 24.0 ± 1.1%, P < 0.05) serine SPG (22.13 ± 2.2 versus 24.9 ± 2.3%, P < 0.01) phosphoglycerides. The treated patients and controls had comparable levels of docosahexaenoic (DHA) and total n-3 fatty acids in EPG and choline phosphoglycerides (CPG). In contrast, the untreated group had significantly (P < 0.05) lower DHA and total n-3 compared with the controls in EPG (2.7 ± 0.4 versus 3.2 ± 0.6% and 4.6 ± 0.5 versus 5.2 ± 0.7%) and CPG (0.7 ± 0.2 versus 1.0 ± 0.2% and 1.2 ± 0.2 versus 1.4 ± 0.3). HU is known to activate cytosolic phospholipase A2 and cyclooxygenase 2, and from this study, it appears to induce mobilisation of AA from the inner cell membrane EPG and SPG. Hence, eicosanoids generated from the released AA may play a role in clinical improvements which occur in HU-treated patients. Hindawi Publishing Corporation 2011 2011-09-15 /pmc/articles/PMC3173880/ /pubmed/21941660 http://dx.doi.org/10.1155/2011/718014 Text en Copyright © 2011 A. A. Daak et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Daak, A. A. Ghebremeskel, K. Elbashir, M. I. Bakhita, A. Hassan, Z. Crawford, M. A. Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease |
title | Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease |
title_full | Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease |
title_fullStr | Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease |
title_full_unstemmed | Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease |
title_short | Hydroxyurea Therapy Mobilises Arachidonic Acid from Inner Cell Membrane Aminophospholipids in Patients with Homozygous Sickle Cell Disease |
title_sort | hydroxyurea therapy mobilises arachidonic acid from inner cell membrane aminophospholipids in patients with homozygous sickle cell disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173880/ https://www.ncbi.nlm.nih.gov/pubmed/21941660 http://dx.doi.org/10.1155/2011/718014 |
work_keys_str_mv | AT daakaa hydroxyureatherapymobilisesarachidonicacidfrominnercellmembraneaminophospholipidsinpatientswithhomozygoussicklecelldisease AT ghebremeskelk hydroxyureatherapymobilisesarachidonicacidfrominnercellmembraneaminophospholipidsinpatientswithhomozygoussicklecelldisease AT elbashirmi hydroxyureatherapymobilisesarachidonicacidfrominnercellmembraneaminophospholipidsinpatientswithhomozygoussicklecelldisease AT bakhitaa hydroxyureatherapymobilisesarachidonicacidfrominnercellmembraneaminophospholipidsinpatientswithhomozygoussicklecelldisease AT hassanz hydroxyureatherapymobilisesarachidonicacidfrominnercellmembraneaminophospholipidsinpatientswithhomozygoussicklecelldisease AT crawfordma hydroxyureatherapymobilisesarachidonicacidfrominnercellmembraneaminophospholipidsinpatientswithhomozygoussicklecelldisease |