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Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection

Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment (SELEX). Low efficiency is one of...

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Detalles Bibliográficos
Autores principales: Shao, Keke, Ding, Weifeng, Wang, Feng, Li, Haiquan, Ma, Da, Wang, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3174225/
https://www.ncbi.nlm.nih.gov/pubmed/21949784
http://dx.doi.org/10.1371/journal.pone.0024910