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Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection
Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment (SELEX). Low efficiency is one of...
Autores principales: | Shao, Keke, Ding, Weifeng, Wang, Feng, Li, Haiquan, Ma, Da, Wang, Huimin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3174225/ https://www.ncbi.nlm.nih.gov/pubmed/21949784 http://dx.doi.org/10.1371/journal.pone.0024910 |
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