Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism

BACKGROUND: Many SNP discrimination strategies employ natural restriction endonucleases to discriminate between allelic states. However, SNPs are often not associated with a restriction site and therefore, a number of attempts have been made to generate sequence-adaptable restriction endonucleases....

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Detalles Bibliográficos
Autores principales: Doyle, Stephen R, Chan, Chee Kai, Grant, Warwick N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175457/
https://www.ncbi.nlm.nih.gov/pubmed/21875442
http://dx.doi.org/10.1186/1472-6750-11-83

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175457/
https://www.ncbi.nlm.nih.gov/pubmed/21875442
http://dx.doi.org/10.1186/1472-6750-11-83

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