Generation of high-titer viral preparations by concentration using successive rounds of ultracentrifugation

BACKGROUND: Viral vectors provide a method of stably introducing exogenous DNA into cells that are not easily transfectable allowing for the ectopic expression or silencing of genes for therapeutic or experimental purposes. However, some cell types, in particular bone marrow cells, dendritic cells a...

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Detalles Bibliográficos
Autores principales: Ichim, Christine V, Wells, Richard A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175463/
https://www.ncbi.nlm.nih.gov/pubmed/21849073
http://dx.doi.org/10.1186/1479-5876-9-137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175463/
https://www.ncbi.nlm.nih.gov/pubmed/21849073
http://dx.doi.org/10.1186/1479-5876-9-137

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