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Generation of high-titer viral preparations by concentration using successive rounds of ultracentrifugation
BACKGROUND: Viral vectors provide a method of stably introducing exogenous DNA into cells that are not easily transfectable allowing for the ectopic expression or silencing of genes for therapeutic or experimental purposes. However, some cell types, in particular bone marrow cells, dendritic cells a...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175463/ https://www.ncbi.nlm.nih.gov/pubmed/21849073 http://dx.doi.org/10.1186/1479-5876-9-137 |