Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes

A markerless genetic exchange system was successfully established in Methanosarcina mazei strain Gö1 using the hpt gene coding for hypoxanthine phosphoribosyltransferase. First, a chromosomal deletion mutant of the hpt gene was generated conferring resistance to the purine analog 8-aza-2,6-diaminopu...

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Detalles Bibliográficos
Autores principales: Ehlers, Claudia, Jäger, Dominik, Schmitz, Ruth A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177094/
https://www.ncbi.nlm.nih.gov/pubmed/21941461
http://dx.doi.org/10.1155/2011/439608

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177094/
https://www.ncbi.nlm.nih.gov/pubmed/21941461
http://dx.doi.org/10.1155/2011/439608

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