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Media composition influences yeast one- and two-hybrid results

Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the me...

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Detalles Bibliográficos
Autores principales: Liu, Ying, Merchant, Zabeena, Hsiao, Hao-Ching, Gonzalez, Kim L, Matthews, Kathleen S, Bondos, Sarah E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177868/
https://www.ncbi.nlm.nih.gov/pubmed/21843345
http://dx.doi.org/10.1186/1480-9222-13-6