Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling

Out of the 90 human protein tyrosine kinases, 81 were assayed with short peptides derived from well-characterized [CDK1(Tyr15), IRS1(Tyr983), and JAK1(Tyr1023)] or generic [polyGlu:Tyr(4:1) and poly-Glu:Ala:Tyr(1:1:1)] substrates. As expected, the CDK1 peptide is a substrate for all Src family kinas...

Descripción completa

Detalles Bibliográficos
Autores principales: Blouin, Julie, Roby, Philippe, Arcand, Mathieu, Beaudet, Lucille, Lipari, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178905/
https://www.ncbi.nlm.nih.gov/pubmed/21966344
http://dx.doi.org/10.2174/1875397301105010115
_version_ 1782212455011713024
author Blouin, Julie
Roby, Philippe
Arcand, Mathieu
Beaudet, Lucille
Lipari, Francesco
author_facet Blouin, Julie
Roby, Philippe
Arcand, Mathieu
Beaudet, Lucille
Lipari, Francesco
author_sort Blouin, Julie
collection PubMed
description Out of the 90 human protein tyrosine kinases, 81 were assayed with short peptides derived from well-characterized [CDK1(Tyr15), IRS1(Tyr983), and JAK1(Tyr1023)] or generic [polyGlu:Tyr(4:1) and poly-Glu:Ala:Tyr(1:1:1)] substrates. As expected, the CDK1 peptide is a substrate for all Src family kinases. On the other hand, some of the activities are novel and lead to a better understanding of the function of certain kinases. Specifically, the CDK1 peptide is a substrate for many of the Eph family members. Interestingly, profiling of nearly all the human protein tyrosine kinases revealed a distinct pattern of selectivity towards the CDK1 and IRS1 peptides.
format Online
Article
Text
id pubmed-3178905
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Bentham Open
record_format MEDLINE/PubMed
spelling pubmed-31789052011-09-30 Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling Blouin, Julie Roby, Philippe Arcand, Mathieu Beaudet, Lucille Lipari, Francesco Curr Chem Genomics Article Out of the 90 human protein tyrosine kinases, 81 were assayed with short peptides derived from well-characterized [CDK1(Tyr15), IRS1(Tyr983), and JAK1(Tyr1023)] or generic [polyGlu:Tyr(4:1) and poly-Glu:Ala:Tyr(1:1:1)] substrates. As expected, the CDK1 peptide is a substrate for all Src family kinases. On the other hand, some of the activities are novel and lead to a better understanding of the function of certain kinases. Specifically, the CDK1 peptide is a substrate for many of the Eph family members. Interestingly, profiling of nearly all the human protein tyrosine kinases revealed a distinct pattern of selectivity towards the CDK1 and IRS1 peptides. Bentham Open 2011-08-22 /pmc/articles/PMC3178905/ /pubmed/21966344 http://dx.doi.org/10.2174/1875397301105010115 Text en © Blouin et al.; Licensee Bentham Open http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Blouin, Julie
Roby, Philippe
Arcand, Mathieu
Beaudet, Lucille
Lipari, Francesco
Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling
title Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling
title_full Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling
title_fullStr Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling
title_full_unstemmed Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling
title_short Catalytic Specificity of Human Protein Tyrosine Kinases Revealed by Peptide Substrate Profiling
title_sort catalytic specificity of human protein tyrosine kinases revealed by peptide substrate profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178905/
https://www.ncbi.nlm.nih.gov/pubmed/21966344
http://dx.doi.org/10.2174/1875397301105010115
work_keys_str_mv AT blouinjulie catalyticspecificityofhumanproteintyrosinekinasesrevealedbypeptidesubstrateprofiling
AT robyphilippe catalyticspecificityofhumanproteintyrosinekinasesrevealedbypeptidesubstrateprofiling
AT arcandmathieu catalyticspecificityofhumanproteintyrosinekinasesrevealedbypeptidesubstrateprofiling
AT beaudetlucille catalyticspecificityofhumanproteintyrosinekinasesrevealedbypeptidesubstrateprofiling
AT liparifrancesco catalyticspecificityofhumanproteintyrosinekinasesrevealedbypeptidesubstrateprofiling

Ejemplares similares