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Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
Fixation ability of five common fixation solutions, including 2.5% glutaraldehyde, 10% formalin, 4% paraformaldehyde, methanol/acetone (1:1), and ethanol/acetic acid (3:1) were evaluated by using atomic force microscopy in the present study. Three model bacteria, i.e., Escherichia coli, Pseudomonas...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Springer Berlin Heidelberg
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181414/ https://www.ncbi.nlm.nih.gov/pubmed/21881891 http://dx.doi.org/10.1007/s00253-011-3551-5 |