Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture

Biphalin is a dimeric opioid peptide that exhibits affinity for three types of opioid receptors (MOP, DOP and KOP). Biphalin is undergoing intensive preclinical study. It was recognized that activation of δ-opioid receptor elicits neuroprotection against brain hypoxia and ischemia. We compare the ef...

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Detalles Bibliográficos
Autores principales: Kawalec, Maria, Kowalczyk, Joanna E., Beresewicz, Malgorzata, Lipkowski, Andrzej W., Zablocka, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2011
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183243/
https://www.ncbi.nlm.nih.gov/pubmed/21842273
http://dx.doi.org/10.1007/s11064-011-0568-1
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author Kawalec, Maria
Kowalczyk, Joanna E.
Beresewicz, Malgorzata
Lipkowski, Andrzej W.
Zablocka, Barbara
author_facet Kawalec, Maria
Kowalczyk, Joanna E.
Beresewicz, Malgorzata
Lipkowski, Andrzej W.
Zablocka, Barbara
author_sort Kawalec, Maria
collection PubMed
description Biphalin is a dimeric opioid peptide that exhibits affinity for three types of opioid receptors (MOP, DOP and KOP). Biphalin is undergoing intensive preclinical study. It was recognized that activation of δ-opioid receptor elicits neuroprotection against brain hypoxia and ischemia. We compare the effect of biphalin and morphine and the inhibition of opioid receptors by naltrexone on survival of neurons in rat organotypic hippocampal cultures challenged with NMDA. Findings: (1) 0.025–0.1 μM biphalin reduces NMDA-induced neuronal damage; (2) biphalin neuroprotection is abolished by naltrexone; (3) reduced number of dead cells is shown even if biphalin is applied with delay after NMDA challenge.
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spelling pubmed-31832432011-10-04 Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture Kawalec, Maria Kowalczyk, Joanna E. Beresewicz, Malgorzata Lipkowski, Andrzej W. Zablocka, Barbara Neurochem Res Original Paper Biphalin is a dimeric opioid peptide that exhibits affinity for three types of opioid receptors (MOP, DOP and KOP). Biphalin is undergoing intensive preclinical study. It was recognized that activation of δ-opioid receptor elicits neuroprotection against brain hypoxia and ischemia. We compare the effect of biphalin and morphine and the inhibition of opioid receptors by naltrexone on survival of neurons in rat organotypic hippocampal cultures challenged with NMDA. Findings: (1) 0.025–0.1 μM biphalin reduces NMDA-induced neuronal damage; (2) biphalin neuroprotection is abolished by naltrexone; (3) reduced number of dead cells is shown even if biphalin is applied with delay after NMDA challenge. Springer US 2011-08-13 2011 /pmc/articles/PMC3183243/ /pubmed/21842273 http://dx.doi.org/10.1007/s11064-011-0568-1 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Kawalec, Maria
Kowalczyk, Joanna E.
Beresewicz, Malgorzata
Lipkowski, Andrzej W.
Zablocka, Barbara
Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
title Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
title_full Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
title_fullStr Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
title_full_unstemmed Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
title_short Neuroprotective Potential of Biphalin, Multireceptor Opioid Peptide, Against Excitotoxic Injury in Hippocampal Organotypic Culture
title_sort neuroprotective potential of biphalin, multireceptor opioid peptide, against excitotoxic injury in hippocampal organotypic culture
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183243/
https://www.ncbi.nlm.nih.gov/pubmed/21842273
http://dx.doi.org/10.1007/s11064-011-0568-1
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