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Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity

To clarify the neuroprotective property of ceruloplasmin and the pathogenesis of aceruloplasminemia, we generated ceruloplasmin-deficient (CP (−/−)) mice on the C57BL/10 genetic background and further treated them with a mitochondrial complex I inhibitor, rotenone. There was no iron accumulation in...

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Autores principales: Hineno, Akiyo, Kaneko, Kazuma, Yoshida, Kunihiro, Ikeda, Shu-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183265/
https://www.ncbi.nlm.nih.gov/pubmed/21706374
http://dx.doi.org/10.1007/s11064-011-0537-8
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author Hineno, Akiyo
Kaneko, Kazuma
Yoshida, Kunihiro
Ikeda, Shu-ichi
author_facet Hineno, Akiyo
Kaneko, Kazuma
Yoshida, Kunihiro
Ikeda, Shu-ichi
author_sort Hineno, Akiyo
collection PubMed
description To clarify the neuroprotective property of ceruloplasmin and the pathogenesis of aceruloplasminemia, we generated ceruloplasmin-deficient (CP (−/−)) mice on the C57BL/10 genetic background and further treated them with a mitochondrial complex I inhibitor, rotenone. There was no iron accumulation in the brains of CP (−/−) mice at least up to 60 weeks of age. Without rotenone treatment, CP (−/−) mice showed slight motor dysfunction compared with CP (+/+) mice, but there were no detectable differences in the levels of oxidative stress markers between these two groups. A low dose of rotenone did not affect the mitochondrial complex I activity in our mice, however, it caused a significant change in motor behavior, neuropathology, or the levels of oxidative stress markers in CP (−/−) mice, but not in CP (+/+) mice. Our data support that ceruloplasmin protects against rotenone-induced oxidative stress and neurotoxicity, probably through its antioxidant properties independently of its function of iron metabolism.
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spelling pubmed-31832652011-10-04 Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity Hineno, Akiyo Kaneko, Kazuma Yoshida, Kunihiro Ikeda, Shu-ichi Neurochem Res Original Paper To clarify the neuroprotective property of ceruloplasmin and the pathogenesis of aceruloplasminemia, we generated ceruloplasmin-deficient (CP (−/−)) mice on the C57BL/10 genetic background and further treated them with a mitochondrial complex I inhibitor, rotenone. There was no iron accumulation in the brains of CP (−/−) mice at least up to 60 weeks of age. Without rotenone treatment, CP (−/−) mice showed slight motor dysfunction compared with CP (+/+) mice, but there were no detectable differences in the levels of oxidative stress markers between these two groups. A low dose of rotenone did not affect the mitochondrial complex I activity in our mice, however, it caused a significant change in motor behavior, neuropathology, or the levels of oxidative stress markers in CP (−/−) mice, but not in CP (+/+) mice. Our data support that ceruloplasmin protects against rotenone-induced oxidative stress and neurotoxicity, probably through its antioxidant properties independently of its function of iron metabolism. Springer US 2011-06-26 2011 /pmc/articles/PMC3183265/ /pubmed/21706374 http://dx.doi.org/10.1007/s11064-011-0537-8 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Hineno, Akiyo
Kaneko, Kazuma
Yoshida, Kunihiro
Ikeda, Shu-ichi
Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity
title Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity
title_full Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity
title_fullStr Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity
title_full_unstemmed Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity
title_short Ceruloplasmin Protects Against Rotenone-Induced Oxidative Stress and Neurotoxicity
title_sort ceruloplasmin protects against rotenone-induced oxidative stress and neurotoxicity
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183265/
https://www.ncbi.nlm.nih.gov/pubmed/21706374
http://dx.doi.org/10.1007/s11064-011-0537-8
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