Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage

RNA polymerase III (pol III) transcription of transfer RNA (tRNA) genes is essential for generating the tRNA adapter molecules that link genetic sequence and protein translation. By mapping pol III occupancy genome-wide in the livers of mouse, rat, human, macaque, dog and opossum, we found that pol...

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Autores principales: Kutter, Claudia, Brown, Gordon D., Gonçalves, Ângela, Wilson, Michael D., Watt, Stephen, Brazma, Alvis, White, Robert J., Odom, Duncan T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184141/
https://www.ncbi.nlm.nih.gov/pubmed/21873999
http://dx.doi.org/10.1038/ng.906
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author Kutter, Claudia
Brown, Gordon D.
Gonçalves, Ângela
Wilson, Michael D.
Watt, Stephen
Brazma, Alvis
White, Robert J.
Odom, Duncan T.
author_facet Kutter, Claudia
Brown, Gordon D.
Gonçalves, Ângela
Wilson, Michael D.
Watt, Stephen
Brazma, Alvis
White, Robert J.
Odom, Duncan T.
author_sort Kutter, Claudia
collection PubMed
description RNA polymerase III (pol III) transcription of transfer RNA (tRNA) genes is essential for generating the tRNA adapter molecules that link genetic sequence and protein translation. By mapping pol III occupancy genome-wide in the livers of mouse, rat, human, macaque, dog and opossum, we found that pol III binding to individual tRNA genes varies substantially in strength and location. However, taking into account tRNA redundancies by grouping pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based isotype classes shows strong conservation. Similarly, pol III occupancy of amino-acid isotypes is almost invariant among transcriptionally and evolutionarily diverse tissues in mouse. Thus, synthesis of functional tRNA isotypes has been highly constrained, though the usage of individual tRNA genes has evolved rapidly.
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spelling pubmed-31841412012-04-01 Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage Kutter, Claudia Brown, Gordon D. Gonçalves, Ângela Wilson, Michael D. Watt, Stephen Brazma, Alvis White, Robert J. Odom, Duncan T. Nat Genet Article RNA polymerase III (pol III) transcription of transfer RNA (tRNA) genes is essential for generating the tRNA adapter molecules that link genetic sequence and protein translation. By mapping pol III occupancy genome-wide in the livers of mouse, rat, human, macaque, dog and opossum, we found that pol III binding to individual tRNA genes varies substantially in strength and location. However, taking into account tRNA redundancies by grouping pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based isotype classes shows strong conservation. Similarly, pol III occupancy of amino-acid isotypes is almost invariant among transcriptionally and evolutionarily diverse tissues in mouse. Thus, synthesis of functional tRNA isotypes has been highly constrained, though the usage of individual tRNA genes has evolved rapidly. 2011-08-28 /pmc/articles/PMC3184141/ /pubmed/21873999 http://dx.doi.org/10.1038/ng.906 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kutter, Claudia
Brown, Gordon D.
Gonçalves, Ângela
Wilson, Michael D.
Watt, Stephen
Brazma, Alvis
White, Robert J.
Odom, Duncan T.
Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage
title Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage
title_full Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage
title_fullStr Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage
title_full_unstemmed Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage
title_short Pol III binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in tRNA gene usage
title_sort pol iii binding in six mammalian genomes shows high conservation among amino acid isotypes, despite divergence in trna gene usage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184141/
https://www.ncbi.nlm.nih.gov/pubmed/21873999
http://dx.doi.org/10.1038/ng.906
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