Stable Isotope Labeling with Amino acids in Nematodes

We describe an approach for the accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) for nematodes, by feeding worms a heavy lysine- and arginine-labeled E. coli strain. We also report a genetic soluti...

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Detalles Bibliográficos
Autores principales: Larance, Mark, Bailly, Aymeric P., Pourkarimi, Ehsan, Hay, Ronald T., Buchanan, Grant, Coulthurst, Sarah, Xirodimas, Dimitris P., Gartner, Anton, Lamond, Angus I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184259/
https://www.ncbi.nlm.nih.gov/pubmed/21874007
http://dx.doi.org/10.1038/nmeth.1679

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184259/
https://www.ncbi.nlm.nih.gov/pubmed/21874007
http://dx.doi.org/10.1038/nmeth.1679

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