Stable Isotope Labeling with Amino acids in Nematodes

We describe an approach for the accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) for nematodes, by feeding worms a heavy lysine- and arginine-labeled E. coli strain. We also report a genetic soluti...

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Detalles Bibliográficos
Autores principales: Larance, Mark, Bailly, Aymeric P., Pourkarimi, Ehsan, Hay, Ronald T., Buchanan, Grant, Coulthurst, Sarah, Xirodimas, Dimitris P., Gartner, Anton, Lamond, Angus I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184259/
https://www.ncbi.nlm.nih.gov/pubmed/21874007
http://dx.doi.org/10.1038/nmeth.1679
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author Larance, Mark
Bailly, Aymeric P.
Pourkarimi, Ehsan
Hay, Ronald T.
Buchanan, Grant
Coulthurst, Sarah
Xirodimas, Dimitris P.
Gartner, Anton
Lamond, Angus I.
author_facet Larance, Mark
Bailly, Aymeric P.
Pourkarimi, Ehsan
Hay, Ronald T.
Buchanan, Grant
Coulthurst, Sarah
Xirodimas, Dimitris P.
Gartner, Anton
Lamond, Angus I.
author_sort Larance, Mark
collection PubMed
description We describe an approach for the accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) for nematodes, by feeding worms a heavy lysine- and arginine-labeled E. coli strain. We also report a genetic solution to remove the arginine-to-proline conversion problem. Combining our approach with quantitative proteomics methods, we characterized the heatshock response in worms.
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spelling pubmed-31842592012-02-28 Stable Isotope Labeling with Amino acids in Nematodes Larance, Mark Bailly, Aymeric P. Pourkarimi, Ehsan Hay, Ronald T. Buchanan, Grant Coulthurst, Sarah Xirodimas, Dimitris P. Gartner, Anton Lamond, Angus I. Nat Methods Article We describe an approach for the accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) for nematodes, by feeding worms a heavy lysine- and arginine-labeled E. coli strain. We also report a genetic solution to remove the arginine-to-proline conversion problem. Combining our approach with quantitative proteomics methods, we characterized the heatshock response in worms. 2011-08-28 /pmc/articles/PMC3184259/ /pubmed/21874007 http://dx.doi.org/10.1038/nmeth.1679 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Larance, Mark
Bailly, Aymeric P.
Pourkarimi, Ehsan
Hay, Ronald T.
Buchanan, Grant
Coulthurst, Sarah
Xirodimas, Dimitris P.
Gartner, Anton
Lamond, Angus I.
Stable Isotope Labeling with Amino acids in Nematodes
title Stable Isotope Labeling with Amino acids in Nematodes
title_full Stable Isotope Labeling with Amino acids in Nematodes
title_fullStr Stable Isotope Labeling with Amino acids in Nematodes
title_full_unstemmed Stable Isotope Labeling with Amino acids in Nematodes
title_short Stable Isotope Labeling with Amino acids in Nematodes
title_sort stable isotope labeling with amino acids in nematodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184259/
https://www.ncbi.nlm.nih.gov/pubmed/21874007
http://dx.doi.org/10.1038/nmeth.1679
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