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Experimental mapping of soluble protein domains using a hierarchical approach

Exploring the function and 3D space of large multidomain protein targets often requires sophisticated experimentation to obtain the targets in a form suitable for structure determination. Screening methods capable of selecting well-expressed, soluble fragments from DNA libraries exist, but require t...

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Detalles Bibliográficos
Autores principales: Pedelacq, Jean-Denis, Nguyen, Hau B., Cabantous, Stephanie, Mark, Brian L., Listwan, Pawel, Bell, Carolyn, Friedland, Natasha, Lockard, Meghan, Faille, Alexandre, Mourey, Lionel, Terwilliger, Thomas C., Waldo, Geoffrey S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185438/
https://www.ncbi.nlm.nih.gov/pubmed/21771856
http://dx.doi.org/10.1093/nar/gkr548