Precise BAC targeting of genetically polymorphic mouse ES cells

The use of bacterial artificial chromosomes (BACs) provides a consistent and high targeting efficiency of homologous recombination in embryonic stem (ES) cells, facilitated by long stretches of sequence homology. Here, we introduce a BAC targeting method which employs restriction fragment length pol...

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Detalles Bibliográficos
Autores principales: Barakat, Tahsin Stefan, Rentmeester, Eveline, Sleutels, Frank, Grootegoed, J. Anton, Gribnau, Joost
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185440/
https://www.ncbi.nlm.nih.gov/pubmed/21737430
http://dx.doi.org/10.1093/nar/gkr550

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185440/
https://www.ncbi.nlm.nih.gov/pubmed/21737430
http://dx.doi.org/10.1093/nar/gkr550

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