An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens

This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR. Supplementary semi-nested human metapneumoviru...

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Detalles Bibliográficos
Autores principales: Chidlow, Glenys R., Harnett, Gerry B., Shellam, Geoffrey R., Smith, David W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185464/
https://www.ncbi.nlm.nih.gov/pubmed/21994537
http://dx.doi.org/10.3390/v1010042

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185464/
https://www.ncbi.nlm.nih.gov/pubmed/21994537
http://dx.doi.org/10.3390/v1010042

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