An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens

This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR. Supplementary semi-nested human metapneumoviru...

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Autores principales: Chidlow, Glenys R., Harnett, Gerry B., Shellam, Geoffrey R., Smith, David W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185464/
https://www.ncbi.nlm.nih.gov/pubmed/21994537
http://dx.doi.org/10.3390/v1010042
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author Chidlow, Glenys R.
Harnett, Gerry B.
Shellam, Geoffrey R.
Smith, David W.
author_facet Chidlow, Glenys R.
Harnett, Gerry B.
Shellam, Geoffrey R.
Smith, David W.
author_sort Chidlow, Glenys R.
collection PubMed
description This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR. Supplementary semi-nested human metapneumovirus and picornavirus PCR assays were required to complete the acute respiratory pathogen profile. Potential pathogens were detected in 85 (70%) of pernasal aspirates collected from 121 children with acute respiratory symptoms. Multiple pathogens were detected in 29 (24%) of those samples. The tandem multiplex real-time PCR was an efficient method for the rapid detection of multiple pathogens.
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spelling pubmed-31854642011-10-12 An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens Chidlow, Glenys R. Harnett, Gerry B. Shellam, Geoffrey R. Smith, David W. Viruses Article This study used real-time PCR assays to screen small sample volumes for a comprehensive range of 35 respiratory pathogens. Initial thermocycling was limited to 20 cycles to avoid competition for reagents, followed by a secondary real-time multiplex PCR. Supplementary semi-nested human metapneumovirus and picornavirus PCR assays were required to complete the acute respiratory pathogen profile. Potential pathogens were detected in 85 (70%) of pernasal aspirates collected from 121 children with acute respiratory symptoms. Multiple pathogens were detected in 29 (24%) of those samples. The tandem multiplex real-time PCR was an efficient method for the rapid detection of multiple pathogens. Molecular Diversity Preservation International (MDPI) 2009-06-08 /pmc/articles/PMC3185464/ /pubmed/21994537 http://dx.doi.org/10.3390/v1010042 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Chidlow, Glenys R.
Harnett, Gerry B.
Shellam, Geoffrey R.
Smith, David W.
An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens
title An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens
title_full An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens
title_fullStr An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens
title_full_unstemmed An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens
title_short An Economical Tandem Multiplex Real-Time PCR Technique for the Detection of a Comprehensive Range of Respiratory Pathogens
title_sort economical tandem multiplex real-time pcr technique for the detection of a comprehensive range of respiratory pathogens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185464/
https://www.ncbi.nlm.nih.gov/pubmed/21994537
http://dx.doi.org/10.3390/v1010042
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