Role of α-Helical Structure in Organic Solvent-Activated Homodimer of Elastase Strain K

Recombinant elastase strain K overexpressed from E. coli KRX/pCon2(3) was purified to homogeneity by a combination of hydrophobic interaction chromatography and ion exchange chromatography, with a final yield of 48% and a 25-fold increase in specific activity. The purified protein had exhibited a fi...

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Detalles Bibliográficos
Autores principales: Rahman, Raja Noor Zaliha Raja Abd., Salleh, Abu Bakar, Basri, Mahiran, Wong, Chee Fah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189751/
https://www.ncbi.nlm.nih.gov/pubmed/22016627
http://dx.doi.org/10.3390/ijms12095797

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189751/
https://www.ncbi.nlm.nih.gov/pubmed/22016627
http://dx.doi.org/10.3390/ijms12095797

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