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Role of α-Helical Structure in Organic Solvent-Activated Homodimer of Elastase Strain K
Recombinant elastase strain K overexpressed from E. coli KRX/pCon2(3) was purified to homogeneity by a combination of hydrophobic interaction chromatography and ion exchange chromatography, with a final yield of 48% and a 25-fold increase in specific activity. The purified protein had exhibited a fi...
Autores principales: | Rahman, Raja Noor Zaliha Raja Abd., Salleh, Abu Bakar, Basri, Mahiran, Wong, Chee Fah |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189751/ https://www.ncbi.nlm.nih.gov/pubmed/22016627 http://dx.doi.org/10.3390/ijms12095797 |
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