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Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes

This study investigates the effects and possible mechanism of an agonist of PPARα, Wy14643, on primary hepatocytes subjected to H/R injury in rats. H/R induced a significant increase ALT, AST, MDA in the culture medium and ROS in the hepatocytes. These effects were reversed by pretreatment with Wy14...

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Autores principales: Chen, Ke, Li, Yuan-Hai, Xu, Si-Qi, Hu, Sheng-Hong, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191773/
https://www.ncbi.nlm.nih.gov/pubmed/22007188
http://dx.doi.org/10.1155/2012/547980
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author Chen, Ke
Li, Yuan-Hai
Xu, Si-Qi
Hu, Sheng-Hong
Zhang, Lei
author_facet Chen, Ke
Li, Yuan-Hai
Xu, Si-Qi
Hu, Sheng-Hong
Zhang, Lei
author_sort Chen, Ke
collection PubMed
description This study investigates the effects and possible mechanism of an agonist of PPARα, Wy14643, on primary hepatocytes subjected to H/R injury in rats. H/R induced a significant increase ALT, AST, MDA in the culture medium and ROS in the hepatocytes. These effects were reversed by pretreatment with Wy14643 in the dose-dependent manner. The activity of SOD and the level of GSH in the hepatocytes were decreased after H/R, which were increased by Wy14643 pretreatment. Moreover, the mRNA expressions of PPARα significantly increased in H/R+Wy14643 groups when compared with that in H/R group. A PPARα agonist, Wy14643, exerts significant protective effect against H/R injury in primary hepatocytes via PPARα activation and attenuating oxidative stress.
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spelling pubmed-31917732011-10-17 Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes Chen, Ke Li, Yuan-Hai Xu, Si-Qi Hu, Sheng-Hong Zhang, Lei PPAR Res Research Article This study investigates the effects and possible mechanism of an agonist of PPARα, Wy14643, on primary hepatocytes subjected to H/R injury in rats. H/R induced a significant increase ALT, AST, MDA in the culture medium and ROS in the hepatocytes. These effects were reversed by pretreatment with Wy14643 in the dose-dependent manner. The activity of SOD and the level of GSH in the hepatocytes were decreased after H/R, which were increased by Wy14643 pretreatment. Moreover, the mRNA expressions of PPARα significantly increased in H/R+Wy14643 groups when compared with that in H/R group. A PPARα agonist, Wy14643, exerts significant protective effect against H/R injury in primary hepatocytes via PPARα activation and attenuating oxidative stress. Hindawi Publishing Corporation 2012 2011-10-09 /pmc/articles/PMC3191773/ /pubmed/22007188 http://dx.doi.org/10.1155/2012/547980 Text en Copyright © 2012 Ke Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Ke
Li, Yuan-Hai
Xu, Si-Qi
Hu, Sheng-Hong
Zhang, Lei
Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes
title Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes
title_full Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes
title_fullStr Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes
title_full_unstemmed Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes
title_short Protective Effects of Peroxisome Proliferator-Activated Receptor-α Agonist, Wy14643, on Hypoxia/Reoxygenation Injury in Primary Rat Hepatocytes
title_sort protective effects of peroxisome proliferator-activated receptor-α agonist, wy14643, on hypoxia/reoxygenation injury in primary rat hepatocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3191773/
https://www.ncbi.nlm.nih.gov/pubmed/22007188
http://dx.doi.org/10.1155/2012/547980
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