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Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer
The highly repetitive Alu retroelements are regarded as methylation centres in the genome. Methylation in the gene promoters could be spreading from them. Promoter methylation of MLH1 is frequently detected in cancers, but the underlying mechanism is unclear. The aim of this study is to understand w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192117/ https://www.ncbi.nlm.nih.gov/pubmed/22022465 http://dx.doi.org/10.1371/journal.pone.0025913 |
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author | Wang, Xiyin Fan, Jing Liu, Dong Fu, Siqing Ingvarsson, Sigurdur Chen, Huiping |
author_facet | Wang, Xiyin Fan, Jing Liu, Dong Fu, Siqing Ingvarsson, Sigurdur Chen, Huiping |
author_sort | Wang, Xiyin |
collection | PubMed |
description | The highly repetitive Alu retroelements are regarded as methylation centres in the genome. Methylation in the gene promoters could be spreading from them. Promoter methylation of MLH1 is frequently detected in cancers, but the underlying mechanism is unclear. The aim of this study is to understand whether the methylation in the Alu elements is associated with promoter methylation in the MLH1 gene. Bisulfite genomic sequencing was used to analyse the CpG sites of the 5′ end (promoter, exon 1 and Alu-containing intron 1) of the MLH1 gene in colorectal cancer cells and tissues, and gastric cancer tissues. Hypomethylation in the Alu elements and hypermethylation in the promoters and the regions between the promoters and the Alu elements were detected in two cancer cell lines and seven cancer tissues. However, demethylation or hypomethylation of the MLH1 promoter and regions between promoter and the Alu elements, and hypermethylation in the Alu elements, were identified in the normal tissues. MLH1 promoter methylation may spread from Alu elements that are located in intron 1 of the MLH1 gene. The trans-acting elements binding to the mutation sites could play a role in the methylation spreading. |
format | Online Article Text |
id | pubmed-3192117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31921172011-10-21 Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer Wang, Xiyin Fan, Jing Liu, Dong Fu, Siqing Ingvarsson, Sigurdur Chen, Huiping PLoS One Research Article The highly repetitive Alu retroelements are regarded as methylation centres in the genome. Methylation in the gene promoters could be spreading from them. Promoter methylation of MLH1 is frequently detected in cancers, but the underlying mechanism is unclear. The aim of this study is to understand whether the methylation in the Alu elements is associated with promoter methylation in the MLH1 gene. Bisulfite genomic sequencing was used to analyse the CpG sites of the 5′ end (promoter, exon 1 and Alu-containing intron 1) of the MLH1 gene in colorectal cancer cells and tissues, and gastric cancer tissues. Hypomethylation in the Alu elements and hypermethylation in the promoters and the regions between the promoters and the Alu elements were detected in two cancer cell lines and seven cancer tissues. However, demethylation or hypomethylation of the MLH1 promoter and regions between promoter and the Alu elements, and hypermethylation in the Alu elements, were identified in the normal tissues. MLH1 promoter methylation may spread from Alu elements that are located in intron 1 of the MLH1 gene. The trans-acting elements binding to the mutation sites could play a role in the methylation spreading. Public Library of Science 2011-10-12 /pmc/articles/PMC3192117/ /pubmed/22022465 http://dx.doi.org/10.1371/journal.pone.0025913 Text en Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Xiyin Fan, Jing Liu, Dong Fu, Siqing Ingvarsson, Sigurdur Chen, Huiping Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer |
title | Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer |
title_full | Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer |
title_fullStr | Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer |
title_full_unstemmed | Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer |
title_short | Spreading of Alu Methylation to the Promoter of the MLH1 Gene in Gastrointestinal Cancer |
title_sort | spreading of alu methylation to the promoter of the mlh1 gene in gastrointestinal cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192117/ https://www.ncbi.nlm.nih.gov/pubmed/22022465 http://dx.doi.org/10.1371/journal.pone.0025913 |
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