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Stretching Actin Filaments within Cells Enhances their Affinity for the Myosin II Motor Domain
To test the hypothesis that the myosin II motor domain (S1) preferentially binds to specific subsets of actin filaments in vivo, we expressed GFP-fused S1 with mutations that enhanced its affinity for actin in Dictyostelium cells. Consistent with the hypothesis, the GFP-S1 mutants were localized alo...
Autores principales: | Uyeda, Taro Q. P., Iwadate, Yoshiaki, Umeki, Nobuhisa, Nagasaki, Akira, Yumura, Shigehiko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192770/ https://www.ncbi.nlm.nih.gov/pubmed/22022566 http://dx.doi.org/10.1371/journal.pone.0026200 |
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