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An overview of enzymatic reagents for the removal of affinity tags
Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Academic Press
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195948/ https://www.ncbi.nlm.nih.gov/pubmed/21871965 http://dx.doi.org/10.1016/j.pep.2011.08.005 |