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An overview of enzymatic reagents for the removal of affinity tags

Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are...

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Detalles Bibliográficos
Autor principal: Waugh, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195948/
https://www.ncbi.nlm.nih.gov/pubmed/21871965
http://dx.doi.org/10.1016/j.pep.2011.08.005