An overview of enzymatic reagents for the removal of affinity tags

Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are...

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Detalles Bibliográficos
Autor principal: Waugh, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195948/
https://www.ncbi.nlm.nih.gov/pubmed/21871965
http://dx.doi.org/10.1016/j.pep.2011.08.005
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author_facet Waugh, David S.
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description Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are needed. Only enzymes have the requisite specificity to be generally useful reagents for this purpose. In this review, the advantages and disadvantages of some commonly used endo- and exoproteases are discussed in light of the latest information.
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spelling pubmed-31959482012-12-01 An overview of enzymatic reagents for the removal of affinity tags Waugh, David S. Protein Expr Purif Review Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are needed. Only enzymes have the requisite specificity to be generally useful reagents for this purpose. In this review, the advantages and disadvantages of some commonly used endo- and exoproteases are discussed in light of the latest information. Academic Press 2011-12 2011-08-19 /pmc/articles/PMC3195948/ /pubmed/21871965 http://dx.doi.org/10.1016/j.pep.2011.08.005 Text en Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Review
Waugh, David S.
An overview of enzymatic reagents for the removal of affinity tags
title An overview of enzymatic reagents for the removal of affinity tags
title_full An overview of enzymatic reagents for the removal of affinity tags
title_fullStr An overview of enzymatic reagents for the removal of affinity tags
title_full_unstemmed An overview of enzymatic reagents for the removal of affinity tags
title_short An overview of enzymatic reagents for the removal of affinity tags
title_sort overview of enzymatic reagents for the removal of affinity tags
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195948/
https://www.ncbi.nlm.nih.gov/pubmed/21871965
http://dx.doi.org/10.1016/j.pep.2011.08.005
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