Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with aldehydes, heavy metal stained using osmium tetroxide and uranyl acetate. They are then dehydrated with...

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Detalles Bibliográficos
Autores principales: Knott, Graham, Rosset, Stéphanie, Cantoni, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196160/
https://www.ncbi.nlm.nih.gov/pubmed/21775953
http://dx.doi.org/10.3791/2588

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196160/
https://www.ncbi.nlm.nih.gov/pubmed/21775953
http://dx.doi.org/10.3791/2588

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