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Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding

Disentangling cellular heterogeneity is a challenge in many fields, particularly in the stem cell and cancer biology fields. Here, we demonstrate how to combine viral genetic barcoding with high-throughput sequencing to track single cells in a heterogeneous population. We use this technique to track...

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Detalles Bibliográficos
Autores principales: Lu, Rong, Neff, Norma F., Quake, Stephen R., Weissman, Irving L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3196379/
https://www.ncbi.nlm.nih.gov/pubmed/21964413
http://dx.doi.org/10.1038/nbt.1977