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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Two electrode voltage clamp electrophysiology (TEVC) is a powerful tool to investigate the mechanism of ion transport1 for a wide variety of membrane proteins including ion channels(2), ion pumps(3), and transporters(4). Recent developments have combined site-specific fluorophore labeling alongside...

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Detalles Bibliográficos
Autores principales: Richards, Ryan, Dempski, Robert E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197104/
https://www.ncbi.nlm.nih.gov/pubmed/21673634
http://dx.doi.org/10.3791/2627