OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons

Focused mutant library generation methods have been developed to improve mainly “localizable” enzyme properties such as activity and selectivity. Current multi-site saturation methods are restricted by the gene sequence, require subsequent PCR steps and/or additional enzymatic modifications. Here we...

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Detalles Bibliográficos
Autores principales: Dennig, Alexander, Shivange, Amol V., Marienhagen, Jan, Schwaneberg, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198389/
https://www.ncbi.nlm.nih.gov/pubmed/22039444
http://dx.doi.org/10.1371/journal.pone.0026222

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198389/
https://www.ncbi.nlm.nih.gov/pubmed/22039444
http://dx.doi.org/10.1371/journal.pone.0026222

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