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OmniChange: The Sequence Independent Method for Simultaneous Site-Saturation of Five Codons
Focused mutant library generation methods have been developed to improve mainly “localizable” enzyme properties such as activity and selectivity. Current multi-site saturation methods are restricted by the gene sequence, require subsequent PCR steps and/or additional enzymatic modifications. Here we...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198389/ https://www.ncbi.nlm.nih.gov/pubmed/22039444 http://dx.doi.org/10.1371/journal.pone.0026222 |