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A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq

The emergence of NextGen sequencing technology has generated much interest in the exploration of transcriptomes. Currently, Illumina Inc. (San Diego, CA) provides one of the most widely utilized sequencing platforms for gene expression analysis. While Illumina reagents and protocols perform adequate...

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Detalles Bibliográficos
Autores principales: Wang, Lin, Si, Yaqing, Dedow, Lauren K., Shao, Ying, Liu, Peng, Brutnell, Thomas P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198403/
https://www.ncbi.nlm.nih.gov/pubmed/22039485
http://dx.doi.org/10.1371/journal.pone.0026426