RMRP Is a Non-Coding RNA Essential for Early Murine Development

RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences fl...

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Detalles Bibliográficos
Autores principales: Rosenbluh, Joseph, Nijhawan, Deepak, Chen, Zhao, Wong, Kwok-Kin, Masutomi, Kenkichi, Hahn, William C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198473/
https://www.ncbi.nlm.nih.gov/pubmed/22039455
http://dx.doi.org/10.1371/journal.pone.0026270
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author Rosenbluh, Joseph
Nijhawan, Deepak
Chen, Zhao
Wong, Kwok-Kin
Masutomi, Kenkichi
Hahn, William C.
author_facet Rosenbluh, Joseph
Nijhawan, Deepak
Chen, Zhao
Wong, Kwok-Kin
Masutomi, Kenkichi
Hahn, William C.
author_sort Rosenbluh, Joseph
collection PubMed
description RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP null mice, indicating that RMRP is essential for early embryonic development.
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spelling pubmed-31984732011-10-28 RMRP Is a Non-Coding RNA Essential for Early Murine Development Rosenbluh, Joseph Nijhawan, Deepak Chen, Zhao Wong, Kwok-Kin Masutomi, Kenkichi Hahn, William C. PLoS One Research Article RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP null mice, indicating that RMRP is essential for early embryonic development. Public Library of Science 2011-10-19 /pmc/articles/PMC3198473/ /pubmed/22039455 http://dx.doi.org/10.1371/journal.pone.0026270 Text en Rosenbluh et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rosenbluh, Joseph
Nijhawan, Deepak
Chen, Zhao
Wong, Kwok-Kin
Masutomi, Kenkichi
Hahn, William C.
RMRP Is a Non-Coding RNA Essential for Early Murine Development
title RMRP Is a Non-Coding RNA Essential for Early Murine Development
title_full RMRP Is a Non-Coding RNA Essential for Early Murine Development
title_fullStr RMRP Is a Non-Coding RNA Essential for Early Murine Development
title_full_unstemmed RMRP Is a Non-Coding RNA Essential for Early Murine Development
title_short RMRP Is a Non-Coding RNA Essential for Early Murine Development
title_sort rmrp is a non-coding rna essential for early murine development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198473/
https://www.ncbi.nlm.nih.gov/pubmed/22039455
http://dx.doi.org/10.1371/journal.pone.0026270
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