Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes

We describe here a method to rapidly convert any desirable DNA fragment, as small as 100 bp, into long tandem DNA arrays up to 140 kb in size that are inserted into a microbe vector. This method includes rolling-circle phi29 amplification (RCA) of the sequence in vitro and assembly of the RCA produc...

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Detalles Bibliográficos
Autores principales: Noskov, Vladimir N, Lee, Nicholas CO, Larionov, Vladimir, Kouprina, Natalay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200152/
https://www.ncbi.nlm.nih.gov/pubmed/21982381
http://dx.doi.org/10.1186/1480-9222-13-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200152/
https://www.ncbi.nlm.nih.gov/pubmed/21982381
http://dx.doi.org/10.1186/1480-9222-13-8

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