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Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes
We describe here a method to rapidly convert any desirable DNA fragment, as small as 100 bp, into long tandem DNA arrays up to 140 kb in size that are inserted into a microbe vector. This method includes rolling-circle phi29 amplification (RCA) of the sequence in vitro and assembly of the RCA produc...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3200152/ https://www.ncbi.nlm.nih.gov/pubmed/21982381 http://dx.doi.org/10.1186/1480-9222-13-8 |