A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors

Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immunoaffinity purification yielded receptors at >90%...

Descripción completa

Detalles Bibliográficos
Autores principales: Corin, Karolina, Baaske, Philipp, Ravel, Deepali B., Song, Junyao, Brown, Emily, Wang, Xiaoqiang, Geissler, Sandra, Wienken, Christoph J., Jerabek-Willemsen, Moran, Duhr, Stefan, Braun, Dieter, Zhang, Shuguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201940/
https://www.ncbi.nlm.nih.gov/pubmed/22039398
http://dx.doi.org/10.1371/journal.pone.0023036

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201940/
https://www.ncbi.nlm.nih.gov/pubmed/22039398
http://dx.doi.org/10.1371/journal.pone.0023036

Ejemplares similares