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Misannotations of rRNA can now generate 90% false positive protein matches in metatranscriptomic studies

In the course of analyzing 9 522 746 pyrosequencing reads from 23 stations in the Southwestern Pacific and equatorial Atlantic oceans, it came to our attention that misannotations of rRNA as proteins is now so widespread that false positive matching of rRNA pyrosequencing reads to the National Cente...

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Detalles Bibliográficos
Autores principales: Tripp, H. James, Hewson, Ian, Boyarsky, Sam, Stuart, Joshua M., Zehr, Jonathan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3203614/
https://www.ncbi.nlm.nih.gov/pubmed/21771858
http://dx.doi.org/10.1093/nar/gkr576