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Gas-phase purification enables accurate, large-scale, multiplexed proteome quantification with isobaric tagging
We describe a mass spectrometry method, QuantMode, which improves the accuracy of isobaric tag–based quantification by alleviating the pervasive problem of precursor interference—co-isolation of impurities—through gas-phase purification. QuantMode analysis of a yeast sample ‘contaminated’ with inter...
Autores principales: | Wenger, Craig D, Lee, M Violet, Hebert, Alexander S, McAlister, Graeme C, Phanstiel, Douglas H, Westphall, Michael S, Coon, Joshua J |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205195/ https://www.ncbi.nlm.nih.gov/pubmed/21963608 http://dx.doi.org/10.1038/nmeth.1716 |
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