Cargando…

Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species

Inflammatory mediators trigger polymorphonuclear neutrophils (PMN) to produce reactive oxygen species (ROS: O(2)(-), H(2)O(2), ∙OH). Mediated by myeloperoxidase in PMN, HOCl is formed, detectable in a chemiluminescence (CL) assay. We have shown that the abundant cytosolic PMN protein calprotectin (S...

Descripción completa

Detalles Bibliográficos
Autores principales: Bøyum, Arne, Skrede, Knut Kristian, Myhre, Oddvar, Tennfjord, Vivi-Ann, Neurauter, Christine Gran, Tolleshaug, Helge, Knudsen, Eirunn, Opstad, Per Kristian, Bjørås, Magnar, Benestad, Haakon B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206613/
https://www.ncbi.nlm.nih.gov/pubmed/22069549
http://dx.doi.org/10.3390/toxins2010095
_version_ 1782215462552076288
author Bøyum, Arne
Skrede, Knut Kristian
Myhre, Oddvar
Tennfjord, Vivi-Ann
Neurauter, Christine Gran
Tolleshaug, Helge
Knudsen, Eirunn
Opstad, Per Kristian
Bjørås, Magnar
Benestad, Haakon B.
author_facet Bøyum, Arne
Skrede, Knut Kristian
Myhre, Oddvar
Tennfjord, Vivi-Ann
Neurauter, Christine Gran
Tolleshaug, Helge
Knudsen, Eirunn
Opstad, Per Kristian
Bjørås, Magnar
Benestad, Haakon B.
author_sort Bøyum, Arne
collection PubMed
description Inflammatory mediators trigger polymorphonuclear neutrophils (PMN) to produce reactive oxygen species (ROS: O(2)(-), H(2)O(2), ∙OH). Mediated by myeloperoxidase in PMN, HOCl is formed, detectable in a chemiluminescence (CL) assay. We have shown that the abundant cytosolic PMN protein calprotectin (S100A8/A9) similarly elicits CL in response to H(2)O(2) in a cell-free system. Myeloperoxidase and calprotectin worked synergistically. Calprotectin-induced CL increased, whereas myeloperoxidase-triggered CL decreased with pH > 7.5. Myeloperoxidase needed NaCl for CL, calprotectin did not. 4-hydroxybenzoic acid, binding ∙OH, almost abrogated calprotectin CL, but moderately increased myeloperoxidase activity. The combination of native calprotectin, or recombinant S100A8/A9 proteins, with NaOCl markedly enhanced CL. NaOCl may be the synergistic link between myeloperoxidase and calprotectin. Surprisingly- and unexplained- at higher concentration of S100A9 the stimulation vanished, suggesting a switch from pro-oxidant to anti-oxidant function. We propose that the ∙OH is predominant in ROS production by calprotectin, a function not described before.
format Online
Article
Text
id pubmed-3206613
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Molecular Diversity Preservation International
record_format MEDLINE/PubMed
spelling pubmed-32066132011-11-08 Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species Bøyum, Arne Skrede, Knut Kristian Myhre, Oddvar Tennfjord, Vivi-Ann Neurauter, Christine Gran Tolleshaug, Helge Knudsen, Eirunn Opstad, Per Kristian Bjørås, Magnar Benestad, Haakon B. Toxins (Basel) Article Inflammatory mediators trigger polymorphonuclear neutrophils (PMN) to produce reactive oxygen species (ROS: O(2)(-), H(2)O(2), ∙OH). Mediated by myeloperoxidase in PMN, HOCl is formed, detectable in a chemiluminescence (CL) assay. We have shown that the abundant cytosolic PMN protein calprotectin (S100A8/A9) similarly elicits CL in response to H(2)O(2) in a cell-free system. Myeloperoxidase and calprotectin worked synergistically. Calprotectin-induced CL increased, whereas myeloperoxidase-triggered CL decreased with pH > 7.5. Myeloperoxidase needed NaCl for CL, calprotectin did not. 4-hydroxybenzoic acid, binding ∙OH, almost abrogated calprotectin CL, but moderately increased myeloperoxidase activity. The combination of native calprotectin, or recombinant S100A8/A9 proteins, with NaOCl markedly enhanced CL. NaOCl may be the synergistic link between myeloperoxidase and calprotectin. Surprisingly- and unexplained- at higher concentration of S100A9 the stimulation vanished, suggesting a switch from pro-oxidant to anti-oxidant function. We propose that the ∙OH is predominant in ROS production by calprotectin, a function not described before. Molecular Diversity Preservation International 2010-01-20 /pmc/articles/PMC3206613/ /pubmed/22069549 http://dx.doi.org/10.3390/toxins2010095 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Bøyum, Arne
Skrede, Knut Kristian
Myhre, Oddvar
Tennfjord, Vivi-Ann
Neurauter, Christine Gran
Tolleshaug, Helge
Knudsen, Eirunn
Opstad, Per Kristian
Bjørås, Magnar
Benestad, Haakon B.
Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species
title Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species
title_full Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species
title_fullStr Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species
title_full_unstemmed Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species
title_short Calprotectin (S100A8/S100A9) and Myeloperoxidase: Co-Regulators of Formation of Reactive Oxygen Species
title_sort calprotectin (s100a8/s100a9) and myeloperoxidase: co-regulators of formation of reactive oxygen species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206613/
https://www.ncbi.nlm.nih.gov/pubmed/22069549
http://dx.doi.org/10.3390/toxins2010095
work_keys_str_mv AT bøyumarne calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT skredeknutkristian calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT myhreoddvar calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT tennfjordviviann calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT neurauterchristinegran calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT tolleshaughelge calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT knudseneirunn calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT opstadperkristian calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT bjørasmagnar calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies
AT benestadhaakonb calprotectins100a8s100a9andmyeloperoxidasecoregulatorsofformationofreactiveoxygenspecies