Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy

We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded...

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Detalles Bibliográficos
Autores principales: Aviles-Espinosa, Rodrigo, Andilla, Jordi, Porcar-Guezenec, Rafael, Olarte, Omar E., Nieto, Marta, Levecq, Xavier, Artigas, David, Loza-Alvarez, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207382/
https://www.ncbi.nlm.nih.gov/pubmed/22076274
http://dx.doi.org/10.1364/BOE.2.003135

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207382/
https://www.ncbi.nlm.nih.gov/pubmed/22076274
http://dx.doi.org/10.1364/BOE.2.003135

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