FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

BACKGROUND: Although a variety of methods and expensive kits are available, molecular cloning can be a time-consuming and frustrating process. RESULTS: Here we report a highly simplified, reliable, and efficient PCR-based cloning technique to insert any DNA fragment into a plasmid vector or into a g...

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Detalles Bibliográficos
Autores principales: Li, Chaokun, Wen, Aiyun, Shen, Benchang, Lu, Jia, Huang, Yao, Chang, Yongchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207894/
https://www.ncbi.nlm.nih.gov/pubmed/21992524
http://dx.doi.org/10.1186/1472-6750-11-92

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3207894/
https://www.ncbi.nlm.nih.gov/pubmed/21992524
http://dx.doi.org/10.1186/1472-6750-11-92

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