Cargando…
Derivation of haploid embryonic stem cells from mouse embryos
Most animals are diploid but haploid-only and male-haplo species have been described (1). Diploid genomes of complex organisms limit genetic approaches in biomedical model species such as in mice. To overcome this problem experimental induction of haploidy has been used in fish (2,3). In contrast, h...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209452/ https://www.ncbi.nlm.nih.gov/pubmed/21900896 http://dx.doi.org/10.1038/nature10448 |
| Sumario: | Most animals are diploid but haploid-only and male-haplo species have been described (1). Diploid genomes of complex organisms limit genetic approaches in biomedical model species such as in mice. To overcome this problem experimental induction of haploidy has been used in fish (2,3). In contrast, haploidy appears less compatible with development in mammals (4,5). Here we describe haploid mouse embryonic stem cells and show their application in forward genetic screening. |
|---|