Derivation of haploid embryonic stem cells from mouse embryos

Most animals are diploid but haploid-only and male-haplo species have been described (1). Diploid genomes of complex organisms limit genetic approaches in biomedical model species such as in mice. To overcome this problem experimental induction of haploidy has been used in fish (2,3). In contrast, h...

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Detalles Bibliográficos
Autores principales: Leeb, Martin, Wutz, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209452/
https://www.ncbi.nlm.nih.gov/pubmed/21900896
http://dx.doi.org/10.1038/nature10448
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author Leeb, Martin
Wutz, Anton
author_facet Leeb, Martin
Wutz, Anton
author_sort Leeb, Martin
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description Most animals are diploid but haploid-only and male-haplo species have been described (1). Diploid genomes of complex organisms limit genetic approaches in biomedical model species such as in mice. To overcome this problem experimental induction of haploidy has been used in fish (2,3). In contrast, haploidy appears less compatible with development in mammals (4,5). Here we describe haploid mouse embryonic stem cells and show their application in forward genetic screening.
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spelling pubmed-32094522012-05-03 Derivation of haploid embryonic stem cells from mouse embryos Leeb, Martin Wutz, Anton Nature Article Most animals are diploid but haploid-only and male-haplo species have been described (1). Diploid genomes of complex organisms limit genetic approaches in biomedical model species such as in mice. To overcome this problem experimental induction of haploidy has been used in fish (2,3). In contrast, haploidy appears less compatible with development in mammals (4,5). Here we describe haploid mouse embryonic stem cells and show their application in forward genetic screening. 2011-09-07 /pmc/articles/PMC3209452/ /pubmed/21900896 http://dx.doi.org/10.1038/nature10448 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Leeb, Martin
Wutz, Anton
Derivation of haploid embryonic stem cells from mouse embryos
title Derivation of haploid embryonic stem cells from mouse embryos
title_full Derivation of haploid embryonic stem cells from mouse embryos
title_fullStr Derivation of haploid embryonic stem cells from mouse embryos
title_full_unstemmed Derivation of haploid embryonic stem cells from mouse embryos
title_short Derivation of haploid embryonic stem cells from mouse embryos
title_sort derivation of haploid embryonic stem cells from mouse embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3209452/
https://www.ncbi.nlm.nih.gov/pubmed/21900896
http://dx.doi.org/10.1038/nature10448
work_keys_str_mv AT leebmartin derivationofhaploidembryonicstemcellsfrommouseembryos
AT wutzanton derivationofhaploidembryonicstemcellsfrommouseembryos

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