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Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies

BACKGROUND: The characterization and cellular localization of transcription factors like NF-κB requires the use of antibodies for western blots and immunohistochemistry. However, if target protein levels are low and the antibodies not well characterized, false positive data can result. In studies of...

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Detalles Bibliográficos
Autores principales: Herkenham, Miles, Rathore, Priyanka, Brown, Pierre, Listwak, Samuel J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210105/
https://www.ncbi.nlm.nih.gov/pubmed/21999414
http://dx.doi.org/10.1186/1742-2094-8-141