Characterization of Family IV UDG from Aeropyrum pernix and Its Application in Hot-Start PCR by Family B DNA Polymerase

Recombinant uracil-DNA glycosylase (UDG) from Aeropyrum pernix (A. pernix) was expressed in E. coli. The biochemical characteristics of A. pernix UDG (ApeUDG) were studied using oligonucleotides carrying a deoxyuracil (dU) base. The optimal temperature range and pH value for dU removal by ApeUDG wer...

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Detalles Bibliográficos
Autores principales: Liu, Xi-Peng, Liu, Jian-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210769/
https://www.ncbi.nlm.nih.gov/pubmed/22087273
http://dx.doi.org/10.1371/journal.pone.0027248

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210769/
https://www.ncbi.nlm.nih.gov/pubmed/22087273
http://dx.doi.org/10.1371/journal.pone.0027248

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