Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers...

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Detalles Bibliográficos
Autores principales: O'Sullivan, Matthew V. N., Zhou, Fei, Sintchenko, Vitali, Kong, Fanrong, Gilbert, Gwendolyn L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3211120/
https://www.ncbi.nlm.nih.gov/pubmed/21847083
http://dx.doi.org/10.3791/2781

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3211120/
https://www.ncbi.nlm.nih.gov/pubmed/21847083
http://dx.doi.org/10.3791/2781

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